TABLE 1.

Properties of subunit associations with glycolipid receptors

Stx B subunit (EC50 [μM] for pentamer formationa)	KD (μM) for binding of Gb3-PC-Ch	Hill coefficient	Bmax
Stx1 B-WT (0.043)	0.089	0.46	0.69
Stx2a B-Q40L (0.11)	0.045	0.33	0.47
Stx2a B-WT (2.3)	0.16	0.75	0.33
Stx2a B subunits with A2 peptide
Stx2a B-WT + A2 peptide	0.038	0.98	0.89
Stx2a B-WT + A2-Stop	0.11	1.2	0.69
Stx2a B-WT + A2-W227R	0.14	0.41	1.4

^aDetermined by the area under the concentration-time curve.