TABLE 1.
Stx B subunit (EC50 [μM] for pentamer formationa) | KD (μM) for binding of Gb3-PC-Ch | Hill coefficient | Bmax |
---|---|---|---|
Stx1 B-WT (0.043) | 0.089 | 0.46 | 0.69 |
Stx2a B-Q40L (0.11) | 0.045 | 0.33 | 0.47 |
Stx2a B-WT (2.3) | 0.16 | 0.75 | 0.33 |
Stx2a B subunits with A2 peptide | |||
Stx2a B-WT + A2 peptide | 0.038 | 0.98 | 0.89 |
Stx2a B-WT + A2-Stop | 0.11 | 1.2 | 0.69 |
Stx2a B-WT + A2-W227R | 0.14 | 0.41 | 1.4 |
Determined by the area under the concentration-time curve.