Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jul 8;7:2041731416656148. doi: 10.1177/2041731416656148

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2016

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages(https://us.sagepub.com/en-us/nam/open-access-at-sage).

PMC Copyright notice

Figure 5. — In vivo angiogenic potential of MAPC and MSC seeded onto demineralized bone matrix (DBM) scaffolds. Representative photomicrographs of formation of new blood vessels in 2-week-old fractures treated with (a) scaffold only, (b) MAPC + DBM scaffold, or (c) MSC + DBM scaffold. Capillaries (arrows) within the developing fracture calluses illustrate more vascularization in fractures treated with MAPC or MSC in addition to the DBM scaffold (*). Hematoxylin and eosin stain, 40× magnification. (d)–(f) von Willebrand Factor (vWF) staining to illustrate vasculature, at 20× magnification. Scaffold-only, MAPC + DBM scaffold, or MSC + DBM scaffold group was implanted into a bone void, and the resultant blood vessel formation was examined after 14 days. Statistically significant blood vessels were noticeable in defects receiving (e) MAPC + DBM scaffold, compared to either (f) MSC + DBM scaffold or (d) scaffold only. (b and e) The blood vessels in the MAPC + DBM scaffold treatment group appeared larger in size and more fully formed or functional (circular). (g) Histological evaluation revealed a significant increase in blood vessel ingrowth in defects treated with MAPC + DBM scaffold compared to MSC + DBM scaffold and scaffold only. Blood vessels were quantified via H&E stained sections (n = 16 sections analyzed for each, *p < 0.05 when compared to scaffold-only control, #p < 0.05 when compared to MAPC + DBM scaffold).