FIG 1.
SPR analysis. Sensorgrams for various concentrations of His-Neph1-CD binding to immobilized Flag-Myo1c-FL are shown. Results are expressed as a differential response (in response units [RU]) versus time. Flag-Myo1c-FL was immobilized on a CM5 chip at 826 RU, and His-Neph1-CD protein at concentrations of 1.0, 0.5, and 0.25 μM was passed over the chip for 3 min and allowed to dissociate for about 4 min. Analysis of the difference between binding sensorgrams of the test and control (without protein) was performed using Biacore BIAevaluation software using a 1:1 Langmuir model. The calculated affinity constant (KD) for Myo1c-Neph1 interaction was 1.1 × 10−8 M (11 nM).