FIG 11.

Neph1 vesicular movement requires its interaction with Myo1c. (A and B) mCherry-Neph1-wt and the K761A mutant were plated on a glass-bottom cell culture plate, and the movement of Neph1-containing vesicles was analyzed using time-lapse live imaging. The vesicular movement was plotted as displacement (micrometers, y axis) versus time (seconds, x axis). (C and D) The displacement (micrometers) and velocity (micrometers per second) of mCherry-Neph1-K761A vesicles were significantly decreased compared to those of mCherry-Neph1-wt (P < 0.001). Data are presented as means ± SEM.