FIG 4.

SAXS data-based shape of the Myo1c-Neph1 complex. (A) Scattering shape of Myo1c-Neph1 complex restored from dummy atom modeling using the SAXS data (I); manual alignment of full-length Myo1c (salmon) on the complex shows an additional volume at the tail region (II and III). (B) The six lowest-energy docking poses of Myo1c-Neph1 complex (I and II); alignment of docking models on SAXS models clearly ruled out two possible docking models (X), and the remaining four could be the more probable structural models of the Myo1c-Neph1 complex (III). The lowest-energy model (√) was used for further interaction analysis (IV).