TABLE 1.
Shape parameters deduced from the SAXS data analysis of proteins and their complexes
| Protein | Mass (kDa) | Indirect Fourier transformation |
Actual concn (mg/ml) | ||
|---|---|---|---|---|---|
| Dmax (Å) | RG (Å) | I0 (AU) | |||
| Standard proteins | |||||
| Lysozyme | 14.2 | 44 | 14.2 | 20 | 1a |
| Gelsolin | 82 | 100 | 30.2 | 115 | 1a |
| Unliganded Myo1c | |||||
| Full length | 168.6 | 220 | 59 | 712 | 3 |
| Myo1c-head | 88.8 | 152 | 49.05 | 215 | 1.7 |
| Myo1c-tail | 37.7 | 157 | 49.7 | 112 | 2.1 |
| Unliganded Neph1-CD and its mutants | |||||
| Neph1-CD | 35.0 | 70 | 21.5 | 120 | 2.4 |
| Neph1-(−)PDZ1 | 34.7 | 70 | 21.1 | 74 | 1.5 |
| Neph1-R750A | 35.0 | 70 | 21.2 | 101 | 2.1 |
| Neph1-K761A | 35.0 | 70 | 21.3 | 96 | 1.9 |
| Neph1-Y762A | 35.0 | 70 | 21.4 | 62 | 1.2 |
a
The values for lysozyme and gelsolin were estimated based on a concentration series of these proteins with predetermined concentrations (for lysozyme, 3 to 5.7 mg/ml, and for gelsolin, 2 to 6 mg/ml) and extrapolated for 1 mg/ml. Based on these standards, the I0 value for 1 kDa of protein at a concentration of 1 mg/ml would be 1.4 AU, and this was used to estimate protein concentrations.