Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Shape parameters and extent of binding deduced from the SAXS data analysis of Myo1c (9.1 μM) with Neph1 and Neph1 mutants
| Protein in complex with Myo1c | Molar ratio of Myo1c to protein | Dmax (Å) | RG (Å) | I0 | Calculated I0 | Extent of binding (%) |
|---|---|---|---|---|---|---|
| Neph1-CD | 1:1.13 | 280 | 76.9 | 523 | 532 | 96 |
| Neph1-(−)PDZ1 | 1:1.14 | 275 | 75 | 525 | 533 | 97 |
| Neph1-R750A | 1:1.12 | 265 | 71.7 | 520 | 529 | 94 |
| Neph1-K761A | 1:1.12 | 225 | 59.7 | 371 | 529 | ∼0 |
| Neph1-Y762A | 1:1.12 | 267 | 68.1 | 522 | 529 | 95 |
| None (buffer) | 183 | 62.8 | 355 | 356 |
