FIG 2.

Identification of global changes in AS after ESRP1/2 depletion. (A) Schematic of ESRP1/2 knockdown experiment in H358 cells using lentiviral shRNAs. (B) Western blot validation of ESRP1/2 protein knockdown. (C) Validation of ESRP1/2 mRNA knockdown by qRT-PCR (from biological triplicates). A one-tailed unpaired t test was used to calculate the P value. The same statistical test was used in all subsequent qRT-PCR analyses whose results are shown in subsequent figures. (D) Heat map for ESRP-regulated skipped exons showed high consistency across replicates. (E) Gene ontology analysis for both EMT-associated and ESRP-regulated SE events showed an enrichment of EMT-relevant processes and pathways.