TABLE 2.

Michaelis constants for wild-type and mutant E2-E3 pairs

E2	Rbx1	Km (μM)
WT Ube2R2	WT	0.15 ± 0.03
WT Ube2R2	R91E	5.40 ± 0.40
WT Ube2D3/UbcH5c	WT	0.33 ± 0.06
WT Ube2D3/UbcH5c	R91E	0.84 ± 0.12
D102K Ube2R2	WT	0.49 ± 0.05
D102K Ube2R2	R91E	1.83 ± 0.27
D103K Ube2R2	WT	0.22 ± 0.04
D103K Ube2R2	R91E	5.79 ± 0.83