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. 2016 May 16;36(11):1691–1703. doi: 10.1128/MCB.01112-15

FIG 5.

FIG 5

San1 promotes chromatin reassembly at the coding sequence. (A to D) ChIP analysis of histone H3 (A and C) and H2B (B and D) levels at the GAL1 coding sequence (toward the 3′ end or ORF2) in the Δsan1 and wild-type strains after switching off transcription in dextrose-containing growth medium (2 [A and B] and 5 [C and D] min). (E to H) ChIP analysis of histone H3 (E [20 min]) and H2B (F [20 min], G [30 min], and H [60 min]) levels at the GAL1 coding sequence in the Δsan1 and wild-type strains after switching on transcription in galactose-containing growth medium for the indicated time periods. (I and J) ChIP analysis of histone H3 (I) and H2B (J) levels at the GAL1 core promoter in the Δsan1 and wild-type strains after switching off transcription in dextrose-containing growth medium (2 min). (K to N) ChIP analysis of histone H3 (K [20 min]) and H2B (L [20 min], M [30 min], and N [60 min]) levels at the GAL1 core promoter in the Δsan1 and wild-type strains after switching on transcription in galactose-containing growth medium for the indicated time periods. (O to R) ChIP analysis of histone H3 and H2B levels at the coding sequences (toward the 3′ end or ORF2 [O and Q]) and core promoters (P and R) of GAL7 (O and P) and GAL10 (Q and R) in the Δsan1 and wild-type strains after switching off transcription in dextrose-containing growth medium (2 min).