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. 2016 May 16;36(11):1691–1703. doi: 10.1128/MCB.01112-15

FIG 6.

FIG 6

High abundance of Spt16 impairs chromatin reassembly at the coding sequence. (A and B) Chromatin reassembly analysis at the GAL1 coding sequence (A) and core promoter (B) following overexpression of Spt16. (C and D) Chromatin reassembly analysis at the GAL10 coding sequence (C) and core promoter (D) following overexpression of Spt16. (E and F) Chromatin reassembly analysis at the GAL7 coding sequence (E) and core promoter (F) following overexpression of Spt16. (G) Growth analysis of the Δsan1 and wild-type strains in the solid YPD medium with or without 0.026% MMS and HU (100 mM) at 30°C. (H) Growth analysis of the yeast strains expressing SPT16 under the control of the GAL1 promoter (PGAL1-SPT16) or of its own endogenous promoter (WT) in the solid YPG medium with or without 0.026% MMS and HU (100 mM) at 30°C. (I) Schematic diagram showing the UPS regulation of Spt16 in controlling chromatin reassembly at the coding sequence and transcriptional elongation.