FIGURE 1.

Growth characteristics of GmCLC1-transgenic Arabidopsis thaliana. (A) Genotyping by PCR of GmCLC1-transgenic A. thaliana lines (L₁–L₅) to confirm success in transformation. All five lines contain the transgene. Comparison in (B) seed germination rate, (C) seedlings growth, and (D) root lengths between A. thaliana wild-type (WT) and transgenic plants (GmCLC1; using L₁ as the representative) sown on MS agar medium containing different concentrations of NaCl. (B) Seeds of WT and GmCLC1 (30 seeds per plate each with three replicates) were surface-sterilized and stratified at 4°C for 2–4 days and then sown on MS medium supplemented with 150 or 200 mM NaCl. After 7 days of incubation, the seed germination rate (%) was measured. (C,D) 5-days-old WT and GmCLC1 with nearly equal root lengths were transferred to MS agar medium supplemented with 0, 150, or 200 mM NaCl. After 5 days of vertical incubation, the seedlings’ growth phenotypes were photographed and the root lengths measured. M: DNA markers. ^∗/^∗∗: The difference between WT and GmCLC1 was significant at p < 0.05 and p < 0.01 respectively, using Duncan’s test. Each bar represents the mean and SD of for (C) 30 seeds per plate each with three replicates; for (D) more than 10 plants for each treatment.