FIGURE 2.

Effects of NaCl treatment on (A) growth, (B) leaf relative water content (RWC), (C) chlorophyll fluorescence (Fv/Fm), and (D) relative electrolyte leakage (REL) in roots and leaves of A. thaliana WT and transgenic GmCLC1 seedlings. Seeds of Arabidopsis WT and transgenic GmCLC1 plants were surface-sterilized and kept at 4°C for 2–4 days and then sown on MS medium. After 8 days of incubation, the seedlings were transferred into plastic pots filled with a sterilized peat moss and vermiculite mixture, and fertilized with ½-strength Hoagland nutrient solution for 5 days in the greenhouse. The nutrient solution was continuously replaced with half-strength Hoagland solution containing 50 mM NaCl for 2 days, followed by 100 mM NaCl for 2 days, and finally 150 mM NaCl for 7 days. No NaCl was added to the nutrient solution for the control treatment. ^∗/^∗∗: The difference between WT and GmCLC1 was significant at p < 0.05/0.01, respectively, using Duncan’s test. Each bar represents the mean and SD of at least three replicates.