FIGURE 3.

Dynamic changes in the chloride (Cl^-) contents in (A) roots and (B) shoots of A. thaliana WT and transgenic GmCLC1 seedlings under 150 mM NaCl stress for 6 days. Seeds of WT and GmCLC1 transgenic A. thaliana were grown on pots containing the sterilized peat moss and vermiculite mixture and grown. After 30 days, plants were treated with 1/2 Hoagland solution containing 150 mM NaCl for 0, 4, 12, 24 h, 2, 4, and 6 days, respectively, then the roots (A) and shoot (B) of Arabidopsis seedlings were sampled and Cl^- contents were measured. ^∗/^∗∗: The difference between WT and the GmCLC1 transgenic line was significant at p < 0.05/0.01, using Duncan’s test. Each bar represents the mean and SD of at least three replicates.