Skip to main content
. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: J Immunol. 2015 Nov 23;196(1):375–384. doi: 10.4049/jimmunol.1501258

Figure 5. IL-21 plays an intrinsic role in KLRG1+ CD8 T cell response.

Figure 5

WT/IL-21R KO mixed bone marrow chimera were generated and CD8 T cell response analyzed at day 14 p.i. A) KLRG1 expression by WT and IL-21R KO CD8 T cells from mixed bone marrow chimera is analyzed by flow cytometry. Plots are gated on total CD8 T cells from WT or IL-21R KO compartment from infected chimeric mice. Bar graphs represent frequency and total number of KLRG1+ cells amongst either WT or IL-21R KO total CD8 T cells. B) Response of KLRG1+ (top row) and KLRG1− (bottom row) CD8 T cells from WT or IL-21R KO compartment from mixed bone marrow chimera is measured by flow cytometry analysis of IFNγ and CD107a staining. Graphs show frequency and total number of IFNγ+, IFNγ+CD107a+ or CD107+ among KLRG1+ CD8 T cells for either WT or IL-21R KO compartments. C) Histogram displays T bet expression by WT and IL-21R KO KLRG1+ CD8 T cells from mixed bone marrow chimera. Graphs represent frequency and MFI for T-bet+ cells for KLRG1+ CD8 T cells from WT and IL-21R KO compartment. Experiments were performed at least twice and data are representative of one experiment.