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. Author manuscript; available in PMC: 2016 Jul 25.
Published in final edited form as: Methods Enzymol. 2015 Sep 11;563:623–642. doi: 10.1016/bs.mie.2015.07.030

Figure 12.

Figure 12

Simulations of the spin label dynamics for the sites observed in Figure 11(A) for monodentate labels and Figure 11(B) for bifunctional label. Position of the oxygen of the nitroxide during the trajectory is shown by the red surface. The binfunctional label shows a dramatic reduction of the available space as compared to the monofunctional, and explains the decreased distance disorder when measuring with the bifunctional label.