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Rotamer checker board of the first three dihedral angles (Cα-Cβ; Cβ-Sγ; Sγ-Sδ), with each square corresponding to a binned rotamer +/−60°. The shading of the squares corresponds to the frequency of the spin label visiting this rotamer conformation while lambda < 0.1 (top); or when the forcefield is fully switched on, lambda=1 (bottom). The scale for those displays is 0–50 for the “visited” display and the fraction of the most prevalent rotamer for the “full forcefield” display.
