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Expression of a heterologous protein in S. boulardii. lacZ from E. coli was overexpressed in both S. boulardii (S. b) and S. cerevisiae by using plasmid p42K-pGPD-lacZ. Transformants of the expression cassette showed β-galactosidase activities compared to control strains without the lacZ expression cassette. ND*, not detected. Results are presented as the mean values and standard deviations of data from three independent biological replicates.
