FIG 3.

Targeted localization of the red fluorescent protein (mRuby) in mitochondria of S. boulardii expressing mRuby with a mitochondrial targeting sequence as indicated by the mitochondrion marker rhodamine 123. The left panel shows differential interference contrast (DIC) images, the second panel from the left shows red fluorescence of mito-mRuby, the third panel from the left shows mitochondrion staining using rhodamine 123, and the right panel shows the overlay of the above-described three images to show the colocalization of mito-mRuby with mitochondria in S. boulardii.