FIG 5.

Human lysozyme is secreted by S. boulardii carrying the integrated human lysozyme gene with the chicken lysozyme signal sequence. (A) Lysozyme activity in concentrated supernatants of S.b(Lys⁻) and S.b(Lys⁺). Results are presented as the mean values and standard deviations of data from three independent biological replicates. (B) Halo assay of concentrated human lysozyme from the fermentation broth of S.b(Lys⁺). Totals of 200 U/ml and 400 U/ml chicken egg white lysozyme were used as controls.