FIG 2.
Peptide inhibition of ISAV infection with an HPR3 strain in ASK cells at 48 h postinfection. The antiviral activity of all peptides was tested using qRT-PCR to analyze the expression of viral segment 8 and the elongation factor 1α (ELF) housekeeping gene in cells. CT values for segment 8 and the ELF gene were used to calculate fold changes in expression of ISAV relative to the untreated-control levels (No Pep). Two peptide concentrations were used to analyze the effect of the dose. The horizontal dashed line indicates the expression level for the untreated control. *, P < 0.01.