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Primers used to amplify the open reading frames from segments 5 and 6 of ISAV and to add restriction sites for cloninga
The segment 8 (Seg8) and elongation factor 1α (ELF) primers were used for gene expression analyses. The −28gIII primer was used for phage-DNA sequencing. The restriction sites for cloning are underlined. FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.
