FIG 1.
mRNA production of genes for biofilm matrix proteins in B. subtilis strain B-1. Gene expression analyses were performed for biofilms generated by wild-type strain B-1, at 10 h (A) and 18 h (B) of biofilm growth. The mRNA production of genes corresponding to matrix components described for wild-type strains NCIB 3610 and B-1, in comparison with the production of the control, 16S rRNA, was analyzed. CT values represent the mean values obtained from 3 biological and 3 technical triplicates. The error bars denote standard deviations. P values from pairwise t tests for these data are given in Table S1 in the supplemental material. Additionally, an ensemble significance test (one-way ANOVA and Tukey's honestly significant difference test; MATLAB) was performed to confirm that the genes blsA, ywsC, epsH, and tasA were transcribed at significantly lower levels than the 16S rRNA control. *, ensemble significant differences.