TABLE 2.
Homology of genes involved in biofilm formation
| Gene(s) | Sequence position (bp)a |
Homology between strains 168 and B-1 (%) | Biofilm matrix component | Reference | |
|---|---|---|---|---|---|
| Strain B-1 | Strain 168 | ||||
| tasA | 3450573 | 2553866 | 75 | Matrix fiber | 48 |
| epsA-O | 2140149 | 3529911 | 78 | Polysaccharide | 42 |
| bslA | 2873830 | 3187503 | 74 | Surface layer protein | 49 |
| tapA | 3452069 | 2555247 | 72 | Lipoprotein (TasA assembly) | 61 |
| sipW | 489452 | 2554502 | 72 | Type I signal peptidase (surface adhesion) | 62 |
| pgsB or ywsC | 3360079 | 3700627 | 82 | γ-Polyglutamate | 51 |
| degU | 628676 | 3645296 | 86 | Two-component response regulator | 63 |
| abrB | 50582 | 45138 | 91 | Transcription factor | 64 |
| sinR | 3450190 | 2552653 | 97 | Master regulator for biofilm formation | 65 |
a
The positions show the start sites of the genes in the respective genomes. Comparisons between the genomes were performed with the NCBI online tools LAST and BLASTx. The recently sequenced B. subtilis strain B-1 (GenBank accession no. CP009684) was compared with the well-studied laboratory strain B. subtilis strain 168 (GenBank accession no. CP010052), which is genetically similar to the wild-type strain NCIB 3610 (55, 66).