FIG 4.

Detection of negative-strand RNA of AcSBV in A. mellifera larvae and adult workers. Lanes 1, 3, and 5, samples obtained on days 2, 6, and 10, respectively; lanes 2, 4, and 6, negative controls without Tag-SB7f primers; lane 7, negative control without the DNA template. Lanes 1, 3, and 5 in panel A represent pooled samples of 6 bees from each of 3 replicates on each day; in panels B, C, and D, results of one replicate are shown. (A) Adult worker bees challenged with AcSBV in the laboratory. (B) Larvae challenged with AcSBV in the laboratory. (C) Adult worker bees challenged with AcSBV in a colony. (D) Larvae challenged with AcSBV in a colony.