TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype/characteristicsa | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| WM3064 | Donor strain for conjugation; ΔdapA | 80 |
| C41(DE3) | Recombinant protein expression host | GE Healthcare |
| S. piezotolerans WP3 | ||
| WP3 | Wild type, GenBank accession no. CP000472 | Lab stock |
| WP3Δhns | WP3 with deletion of the hns gene | This work |
| WP3Δhns-C | WP3Δhns with pSW2-hns | This work |
| Plasmids | ||
| pRE112 | Allelic-exchange vector; Cmr sacB | 48 |
| pSW2 | Shuttle vector for complementation; Cmr, derived from the filamentous bacteriophage SW1 | 81 |
| pET28a | Kanr, His-tag protein expression vector | GE Healthcare |
| pRE112-hns | pRE112 containing the PCR fragment for deletion of the hns gene | This work |
| pSW2-hns | pSW2 containing hns and its upstream promoter region | This work |
| pET28a-hns | pET28a containing the coding region of the hns gene | This work |
a
Cmr, chloramphenicol resistance; Kanr, kanamycin resistance; GST, glutathione S-transferase.