TABLE 2.
Primer name | Sequence (5′-3′) | Description |
---|---|---|
hnsUR | GGCTCTAGAGGCCAATACTGAGTACAACAAC | hns deletion |
hnsDL | TGGGTTCTTAAAGGGCGTCCGATTAAAAAAAATTGATAAAGG | hns deletion |
hnsDR | GCCCTTTAAGAACCCAGCATTTGCT | hns deletion |
Chlfor | ATGCCCGGGCTCTGAATGGATAGCGCCAT | Mutant conformation |
Chlrev | TTTACCAGAGGTCGCAGAA | Mutant conformation |
hnsCFor | CAGCCAAGTATAGTCACCAATT | Mutant complementation |
hnsCRev | ATACACGCGTAGGGCTTCCTTCTTACGTCTCCCAGCTCGAGCTGGGTTCTTAAAGGGCAAA | Mutant complementation |
HNSexpFor | ACTTAGGATCCATGAGCGAATTTTTAGATATTTTG | H-NS expression |
HNSexpRev | AAAGAGAGCTCTTAGATAAGGAAGTCTTCCATAGT | H-NS expression |
PmotYFor | GACAACTTAATGACGCAG | EMSA |
PmotYRev | TCCTCAGTTTTATCCCTT | EMSA |
PlafBFor | TTCTTCCCCCCACCCAATTT | EMSA |
PlafBRev | TACGATCCTCCAATCTAGCT | EMSA |
PlafAFor | GTTCAGTCTCTTTACGCTTA | EMSA |
PlafARev | AACGTGTTCCTTTAGGCTTT | EMSA |
PmotY-DF-For | 6-FAM-GACAACTTAATGACGCAG | DNase I footprinting |
PlafB-DF-For | 6-FAM-TTCTTCCCCCCACCCAATTT | DNase I footprinting |
lafARTFor | AAACAGCCAGCCGTAACGTT | qPCR |
lafARTRev | TGCACCATCTGCAGTTTGGA | qPCR |
fliA2RTFor | TTTTGGCCATCGAAGACATG | qPCR |
fliA2RTRev | GCCTTGCGGACTCGAGTAAC | qPCR |
pepNRTFor | TTAAGGCAATGGAAGCTGCAT | qPCR |
pepNRTRev | CGTCTTTACCCGTTAATGATACGA | qPCR |
swp3093RTFor | GATGTTGCGTACGCGATGTC | qPCR |
swp3093RTRev | TCTGCATAGCCTGTTAAGTCCAAA | qPCR |
swp0265RTFor | TGGCGAGCATGTCACTACAGA | qPCR |
swp0265RTRev | GGGCTGATTTGCCATCCA | qPCR |
swp2979RTFor | TTTGCTGTCGTTGCAGATGAG | qPCR |
swp2979RTRev | TCAGTCGATTCTTGCGTTCGT | qPCR |
swp5089RTFor | GCTTCAGGCAACCGCATAG | qPCR |
swp5089RTRev | TGCAACCTCATCAGCACGTT | qPCR |
swp4459RTFor | CTTGGTCGTTGGAGCTGTACAG | qPCR |
swp4459RTRev | CCGCAGTATTCCCCACCATAT | qPCR |
swp2844RTFor | CGGATTTGAGCGCACGTT | qPCR |
swp2844RTRev | CAAGATCTGCGCCAATGACA | qPCR |