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. 2016 Apr 4;82(8):2388–2398. doi: 10.1128/AEM.00297-16

TABLE 2.

Primers used in this study

Primer name Sequence (5′-3′) Description
hnsUR GGCTCTAGAGGCCAATACTGAGTACAACAAC hns deletion
hnsDL TGGGTTCTTAAAGGGCGTCCGATTAAAAAAAATTGATAAAGG hns deletion
hnsDR GCCCTTTAAGAACCCAGCATTTGCT hns deletion
Chlfor ATGCCCGGGCTCTGAATGGATAGCGCCAT Mutant conformation
Chlrev TTTACCAGAGGTCGCAGAA Mutant conformation
hnsCFor CAGCCAAGTATAGTCACCAATT Mutant complementation
hnsCRev ATACACGCGTAGGGCTTCCTTCTTACGTCTCCCAGCTCGAGCTGGGTTCTTAAAGGGCAAA Mutant complementation
HNSexpFor ACTTAGGATCCATGAGCGAATTTTTAGATATTTTG H-NS expression
HNSexpRev AAAGAGAGCTCTTAGATAAGGAAGTCTTCCATAGT H-NS expression
PmotYFor GACAACTTAATGACGCAG EMSA
PmotYRev TCCTCAGTTTTATCCCTT EMSA
PlafBFor TTCTTCCCCCCACCCAATTT EMSA
PlafBRev TACGATCCTCCAATCTAGCT EMSA
PlafAFor GTTCAGTCTCTTTACGCTTA EMSA
PlafARev AACGTGTTCCTTTAGGCTTT EMSA
PmotY-DF-For 6-FAM-GACAACTTAATGACGCAG DNase I footprinting
PlafB-DF-For 6-FAM-TTCTTCCCCCCACCCAATTT DNase I footprinting
lafARTFor AAACAGCCAGCCGTAACGTT qPCR
lafARTRev TGCACCATCTGCAGTTTGGA qPCR
fliA2RTFor TTTTGGCCATCGAAGACATG qPCR
fliA2RTRev GCCTTGCGGACTCGAGTAAC qPCR
pepNRTFor TTAAGGCAATGGAAGCTGCAT qPCR
pepNRTRev CGTCTTTACCCGTTAATGATACGA qPCR
swp3093RTFor GATGTTGCGTACGCGATGTC qPCR
swp3093RTRev TCTGCATAGCCTGTTAAGTCCAAA qPCR
swp0265RTFor TGGCGAGCATGTCACTACAGA qPCR
swp0265RTRev GGGCTGATTTGCCATCCA qPCR
swp2979RTFor TTTGCTGTCGTTGCAGATGAG qPCR
swp2979RTRev TCAGTCGATTCTTGCGTTCGT qPCR
swp5089RTFor GCTTCAGGCAACCGCATAG qPCR
swp5089RTRev TGCAACCTCATCAGCACGTT qPCR
swp4459RTFor CTTGGTCGTTGGAGCTGTACAG qPCR
swp4459RTRev CCGCAGTATTCCCCACCATAT qPCR
swp2844RTFor CGGATTTGAGCGCACGTT qPCR
swp2844RTRev CAAGATCTGCGCCAATGACA qPCR