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Setup for the incubation of Beggiatoa sp. 35Flor in the presence of diffusional gas gradients. The Beggiatoa mat grows between opposed diffusional gradients of oxygen, sulfide, and (if the latter is provided) hydrogen. Oxygen is supplied to the mat by diffusion from the headspace above, and sulfide is provided by diffusion from the bottom agar below. Hydrogen can be added to the gas reservoir and can reach the mat by diffusion through the silicone plug.
