FIG 5.
Detection of B. anthracis spores in soil. B. anthracis spores (1.3 × 105 to 1.3 × 107 CFU/g) were inoculated into soil (1.0 g) and maintained overnight (∼16 h) at 4°C before being mixed with Wβ::luxAB-2 (final concentration, 4 × 107 PFU/ml) and incubated in TSB (containing 0.1 M l-alanine) at 35°C. Bioluminescence was measured 1.5, 3, and 6 h following the addition of n-decanal. Values represent the means ± SD (n = 3). *, P < 0.05 (two-way ANOVA) for results compared to those with phage-only controls.