FIG 1.

Adaptation of T. kivui to growth on CO. (A) A culture adapted to growth on H₂ plus CO₂ served as the preculture for inoculation of fresh medium in the absence of CO (■) or in the presence of 10% CO (●) or 30% CO (○). (B) The culture cultivated on 10% CO from panel A served as the preculture to reinoculate fresh medium in the absence of CO (■) or in the presence of 10% CO (●) or 20% CO (○). (C) The 20% CO culture from panel B was used as the preculture for cultivation on 30% CO (□), which in turn served as the preculture for cultivation on 40% CO (△). The latter served as the preculture for growth on 50% CO (▼). N₂ plus CO₂ (80:20 [vol/vol]) was used as the makeup gas at a final pressure of 2 × 10⁵ Pa. All cultures were grown in complex medium, and growth was measured by monitoring the optical density at 600 nm. Shown are data from one representative experiment out of three independent replicates.