TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Lactococcus lactis strains | ||
| LMG2081 | Bacteriocin (lactococcin G and lacticin LMG) producer | 19 |
| BGBM50 | Lactococcin G producer | 37 |
| BGMN1-596 | Plasmid-free derivate of L. lactis subsp. lactis BGMN1-5 | 35 |
| LMG2081/pG+host9lcnG | LcnG−, LctLMG+, LctLMGr | This work |
| LMG2081/pG+host9lctLMG | LcnG+, LctLMG−, LcnGr | This work |
| Escherichia coli strains | ||
| DH5α | λ− Ф80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK−) supE44 thi-1 gyrA relA1 | 38 |
| EC101 | JM101 containing repA gene of pWV01 in chromosome | 39 |
| DH5α/pGEM-T EasylcnG | This work | |
| DH5α/pGEM-T EasylctLMG | This work | |
| DH5α/pGEM-T EasyLL/LD | This work | |
| EC101/pG+host9lcnG | This work | |
| EC101/pG+host9lctLMG | This work | |
| Plasmids | ||
| pG+host9 | Emr; thermosensitive vector | 40 |
| pG+host9lcnG | pG+host9 carrying fragment of lcnG gene | This work |
| pG+host9lctLMG | pG+host9 carrying fragment of lmgM gene | This work |
| pGEM-T Easy | 3,015 bp; Ampr; PCR cloning vector | Promega |
| pGEM-T EasylcnG | This work | |
| pGEM-T EasylctLMG | pGEM-T Easy carrying 814 bp of lmgM gene | This work |
| ppGEM-T EasyLL/LD | This work |