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. 2016 Jul 22;3(5):e263. doi: 10.1212/NXI.0000000000000263

Figure 1. Detection of KIR4.1 autoantibodies using ELISA with the synthetic peptide.

Figure 1.

(A) To ensure that each well was coated with the synthetic peptide, the ELISA assay was performed with anti-KIR4.192–105 rabbit antiserum. The antibody bound in a dose-dependent manner. The x-axis indicates the amount of rabbit antiserum for the KIR4.192–105 peptide used. The lines with open and closed circles are the results using preimmune serum and antiserum for the KIR4.192–105 peptide, respectively. The y-axis indicates the optical density at 450 nm. (B) No patient with MS was positive for the antibody (0%). No patient was positive for the antibody among 40 patients with anti-aquaporin-4 antibody–positive NMO spectrum disorder (0%). There were no positive samples in the HC group. HC = healthy controls; KIR4.1 = inward rectifying potassium channel 4.1; MS = multiple sclerosis; NMO = neuromyelitis optica.