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ET helps to properly identify elements of the junctional sarcoplasmic reticulum (jSR) network. (A) Three seemingly unconnected membranous structures (arrows) are demonstrated to be connected further down the Z-planes. Scale bar = 200 nm. (B) Example of jSR (blue) and T-tub (green) manual segmentation along the Z-plane and a resulting 3D meshed model showing close spatial relationship of the two structures. Scale bar = 200 nm.
