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Proper identification of microtubules (MT) in TEM data is hindered by their often deceptive appearance that depends on their orientation relative to the cutting plane. (A) A schematic representation of the appearance of a microtubule in 2D section depending on its orientation relative to the cutting plane. (B) MT that may be missed in the native cutting plane (top) can be identified (arrows) and followed in 3D tomograms using ‘image rotation tools. Scale bar = 200 nm.
