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The expression of RipAY, but not RipAY E216Q, induces GSH depletion in yeast cells. The GSH level in yeast cells expressing RipAY (solid circles), RipAY E216Q (open circles), and YER163c (solid triangles) was measured every 2 h after induction. The relative GSH levels (in percentages) are shown in comparison with the vector control. The data are means ± standard deviations (SD) from three independent experiments.
