FIG 5 .

Extended designer cellulosomes. (A) Schematic representation of the extension of the designer cellulosome concept. The previously described hexavalent scaffoldin with its 6 enzymes (1) is supplemented with 2 additional enzymes using the adaptor strategy, as follows: Adaptor1 integrates 3 cellulases, a-9A, b-48A, and 5A-t, and attaches to Scaf4, which incorporates 5 additional enzymes, including 4 xylanases, 43A-c, 11A-a, 10B-t, and 10A-f, and one cellulase, 6A-g. (B) Comparative degradation of wheat straw after 48 h by the chimeric cellulosomal enzymatic complexes and free chimeric and wild-type enzymes indicated in the respective columns in panel C below. Enzymatic activities, measured in total reducing sugars released (µM) are shown. Error bars show standard deviations. (C) Contents of enzymes and scaffoldins in the various samples. The enzymatic system summarized in column 1 contains only 6 chimeric enzymes, 43A-c, 11A-a, 10B-t, 6A-g, 10A-f, and b-48A, while in the systems summarized in columns 2 to 7, two additional enzymes are included, a-9A and 5A-t, to complete a total of 8 enzymes. The system summarized in column 8 is a mixture of the 8 equivalent wild-type T. fusca enzymes (free native system).