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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: Biochem Pharmacol. 2015 Sep 28;98(4):649–658. doi: 10.1016/j.bcp.2015.09.015

Figure 2. Na-fluorescein uptake in Sf9 cells overexpressing human OATP1B1 or 1B3.

Figure 2

A Histograms show the fluorescence of 1 μM Na-Fluo in Sf9 cells expressing an unrelated protein (control), OATP1B1 or OATP1B3 in the presence or absence of 20 μM cyclosporin A (CsA). The result of 1 representative experiment is shown.

B 1 μM Na-Fluo accumulation was measured for 0.5-20 minutes at 37°C and pH 7.4. Geomean fluorescence in 1B1 or 1B3-overexpressing insect cells (minus background fluorescence measured in unrelated virus-infected cells) was determined by flow cytometry. Data points represent the average of three independent experiments +/− SD values.

C Accumulation of various concentrations (see axis x) of Na-Fluo after incubation for 4 minutes at 37°C and pH 7.4 as measured by flow cytometry. Geomean values of three independent experiments +/− SD values are shown.

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