Table 1.
qPCR probes and primer sequences
| Target | mRNA Primer Sequence 5’-3’ | GenBank Accession | |
|---|---|---|---|
| β-actin | β-actin-F: TCCTTCCTGGGCATGGAATC | ||
| β-actin-R: GGGCGCGATGATCTTGATCT | U39357 | ||
| β-actin-Probe (FAM labelled): CCTTCCTTCCTGGGCATGGAATCC | |||
| Galanin | GAL-F: GAGAGGCTGGACCCTGAACA | ||
| GAL-R: CGTGAAATGACCTGTGGTTGTC | EF192581 | ||
| GAL-Probe (FAM labelled): TGCCGGCTACCTTCTCGGACCA | |||
| GALR1 | GALR1-F: CACACCACGTAGGCCTTCTTG | ||
| GALR1-R: GACGTCAGCAACCAGACCTTCT | EF192582 | ||
| GALR1-Probe (FAM labelled): CGCTGGTTGGGCCACTGCTCC | |||
| GALR2 | GALR2-F: AGCCGTCCAGGGTGTAGATG | ||
| GALR2-R: CGACCTGTGTTTCATCGTGTG | EF192583 | ||
| GALR2-Probe (FAM labelled): CTTGGAAGGGCACGCAG | |||
| GALR3 | GALR3-F: ACGACGGATCTATTCATACCTCAAC | ||
| GALR3-R: GATGGCGGCCTGGAAAG | EF192584 | ||
| GALR3-Probe (FAM labelled): CTGGCGGCAGCTGACCTCTGCT | |||
| GnRH | GnRH-F: GGTCGATCAGCCAGTAGAACCT | ||
| GnRH-R: AGGTCCCTCAGAGGAGAATGG | U02517 | ||
| GnRH-Probe (FAM labelled): TGGTGAACAATGCACCCACAGCACT | |||
| GnRHR | GnRHR-F: TGTAACACACTGCAGTTTTCCACA | ||
| GnRHR-R: AAGAGGGATGATGAAGAGGCAG | L22215 | ||
| GnRHR-Probe (FAM labelled): TGGCATCAAGCCTTTTATAACTTTTTCACCTTCA | |||