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. 2016 Jul 20;9:4453–4464. doi: 10.2147/OTT.S105664

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© 2016 Chen et al. This work is published and licensed by Dove Medical Press Limited

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The RNAi-mediated downregulation of autophagy-related genes hinders lapatinib-induced autophagic cell death in U937 cells.

Notes: U937 cells with shRNA expression were prepared. Cells were transduced with shRNA against ATG5, 7, Beclin-1 (BECN), and RFP (nonspecific) mRNA, selected with puromycin, and treated with DMSO(D) or 5–10 µM lapatinib for 2 days. The relative percentages of survival rates were detected using the MTS (A) and trypan blue (B) assays. Two shATG7 or shBECN clones (#1 or #2) were used. *P<0.05, **P<0.01, and ***P<0.001 (Student’s t-test) as compared to the DMSO control.

Abbreviations: RNAi, RNA interference; shRNA, short hairpin RNA; DMSO, dimethyl sulfoxide; MTS, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; Unif, uninfected cells.