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. 2016 Jul 20;9:4453–4464. doi: 10.2147/OTT.S105664

Figure 6.

Figure 6

The upregulation of CD14 and CD68 macrophagic differentiation markers and the phagocytosis ability of lapatinib in AML U937 leukemia cells.

Notes: (A, B) U937 cells were treated with DMSO, 5–10 µM lapatinib, or 0.1 µM TPA (differentiation inducer) for 3 days. Cells were harvested, stained with antibodies against CD14 or CD68, and positive cells were analyzed by flow cytometry. (C–E) U937 cells were treated with DMSO, 2.5–10 µM lapatinib, or 0.1 µM TPA (differentiation inducer) as indicated for 3 days. Cells were harvested, incubated with fluorescent latex beads (C, D) or DCFH-DA (E) for the detection of the phagocytosis ability of cells (C, D) or the ROS production of cells (E) by flow cytometry. (D) MFI in (C) is expressed. ***P<0.001 (t-test) as compared to the DMSO control.

Abbreviations: AML, acute myeloid leukemia; DMSO, dimethyl sulfoxide; TPA, 12-O-tetradecanoylphorbol-13-acetate; DCFH-DA, 2′,7′-dichlorofluorescein diacetate; ROS, reactive oxygen species; MFI, mean fluorescence intensity.