Figure 5.

Suppressor of cytokine signaling 3–knockout (SOCS-3-KO) regulates cytokine production by peritoneal macrophages stimulated with Porphyromonas gingivalis lipopolysaccharide (LPS). Peritoneal macrophages isolated from wild-type (WT) and myeloid lineage–specific SOCS-3-KO mice were incubated with either culture medium alone (control) or 100 ng/mL of P. gingivalis LPS for 12 h. The levels of IL-1β, IL-6, and KC (IL-8) in the culture supernatants were measured with Luminex multiplex bead immunoassay. A significantly higher secretion of IL-1β, IL-6, and KC (IL-8) by macrophages from myeloid SOCS-3-KO mice was observed when compared with macrophages from WT ones after P. gingivalis LPS stimulation (P < 0.001). No significant differences in the cytokine levels between control samples of different mouse genotype groups were observed (P > 0.05). The values presented are the mean ± SD (n = 6 wells per group).