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. 2016 May 4;95(9):1042–1048. doi: 10.1177/0022034516645389

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© International & American Associations for Dental Research 2016

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Figure 3. — Acceptor photobleaching fluorescence resonance energy transfer images of transverse section of mouse mandibular molar at postnatal day 8 (P8). (A) Donor emission channel, amelogenin (Amel) stained with anti-chicken-FITC secondary antibody. (B) Acceptor emission channel, ameloblastin (Ambn) stained with anti-goat-TRITC secondary antibody. (C) Overlay of donor and acceptor channels. (A1, B1, C1) Magnified images of donor and acceptor channels and their overlay, which were acquired sequentially before acceptor photobleaching. (A2, B2, C2) Magnified images after acceptor photobleaching. The regions chosen for photobleaching are represented by white boxes in B1 and B2. The height and width of the boxes range from 7 to 12 μm and 10 to 14 μm, respectively. Am, ameloblast; D, dentine; EM, enamel matrix; FITC, fluorescein isothiocyanate; ROI, region of interest; TRITC, tetramethylrhodamine.