TABLE 1 .
Saccharomyces cerevisiae strains used in this study
| Strain | Relevant genotypea | Parental strain(s) | Source or reference |
|---|---|---|---|
| CEN.PK113-7D | MATa | P. Kötter | |
| IMX585 | MATa can1Δ::cas9-natNT2 | CEN.PK113-7D | 33 |
| IMX719 | MATa can1Δ::cas9-natNT2 acs1Δ acs2Δ::{PDHL} | IMX585 | 33 |
| IMX868 | MATα can1Δ::cas9-natNT2 sga1Δ::{CARN} | 8 | |
| IMX745 | MATa can1Δ::cas9-natNT2 acs1Δ acs2Δ::{PDHL} sga1Δ::{CARN} | IMX719 | This study |
| IMS0482 | MATa can1Δ::cas9-natNT2 acs1Δ acs2Δ::{PDHL} sga1Δ::{CARN} | IMX745 | This study |
| IMS0483 | MATa can1Δ::cas9-natNT2 acs1Δ acs2Δ::{PDHL} sga1Δ::{CARN} | IMX745 | This study |
| IMW074 | MATa can1Δ::cas9-natNT2 acs1Δ acs2Δ::{PDHL} sga1Δ | IMS0482 | This study |
| IMW075 | MATa can1Δ::cas9-natNT2 acs1Δ acs2Δ sga1Δ::{CARN} | IMS0482 | This study |
| IMW076 | MATa can1Δ::cas9-natNT2 acs1Δ acs2Δ::{PDHL} sga1Δ | IMS0483 | This study |
| IMW077 | MATa can1Δ::cas9-natNT2 acs1Δ acs2Δ sga1Δ::{CARN} | IMS0483 | This study |
| IMW078 | MATa can1Δ::cas9-natNT2 acs1Δ acs2Δ::{PDHL} sga1Δ::{CARN} ach1Δ | IMS0482 | This study |
| IMW079 | MATa can1Δ::cas9-natNT2 acs1Δ acs2Δ::{PDHL} sga1Δ::{CARN} pda1Δ | IMS0482 | This study |
| IMW081 | MATa can1Δ::cas9-natNT2 acs1Δ acs2Δ::{PDHL} sga1Δ::{CARN} ach1Δ | IMS0483 | This study |
| IMW082 | MATa can1Δ::cas9-natNT2 acs1Δ acs2Δ::{PDHL} sga1Δ::{CARN} pda1Δ | IMS0483 | This study |
| IMX847 | MATa can1Δ::cas9-natNT2 acs1Δ acs2Δ::{PDHL} sga1Δ::{CARN} MCT1T641G | IMX745 | This study |
| IMX849 | MATa can1Δ::cas9-natNT2 acs1Δ acs2Δ::{PDHL} sga1Δ::{CARN} RTG2G503T | IMX745 | This study |
| IMX852 | MATa can1Δ::cas9-natNT2 acs1Δ acs2Δ::{PDHL} sga1Δ::{CARN} MCT1T641G RTG2G503T | IMX745 | This study |
| IMX907 | MATa can1Δ::cas9-natNT2 acs1Δ acs2Δ::{PDHL} sga1Δ::{CARN, pADH1-YAT2C173G} | IMX745 | This study |
| IMX909 | MATa can1Δ::cas9-natNT2 acs1Δ acs2Δ::{PDHL} sga1Δ::{CARN,pADH1-YAT2C173G} MCT1T641G | IMX847 | This study |
| IMX911 | MATa can1Δ::cas9-natNT2 acs1Δ acs2Δ::{PDHL} sga1Δ::{CARN,pADH1-YAT2C173G} RTG2G503T | IMX849 | This study |
| IMX913 | MATa can1Δ::cas9-natNT2 acs1Δ acs2Δ::{PDHL} sga1Δ::{CARN,pADH1-YAT2C173G} MCT1T641G RTG2G503T | IMX852 | This study |
| IMX932 | MATa can1Δ::cas9-natNT2 acs1Δ acs2Δ::{PDHL} sga1Δ::{CARN,yat2Δ} MCT1T641G RTG2G503T | IMX852 | This study |
| IMX933 | MATa can1Δ::cas9-natNT2 acs1Δ acs2Δ::{PDHL} sga1Δ::{CARN,pADH1-YAT2C173G} MCT1T641G rtg2Δ | IMX909 | This study |
| IMX934 | MATa can1Δ::cas9-natNT2 acs1Δ acs2Δ::{PDHL} sga1Δ::{CARN,pADH1-YAT2C173G} mct1Δ RTG2G503T | IMX911 | This study |
| IMX923 | MATa can1Δ::cas9-natNT2 sga1Δ::pADH1-YAT2-tYAT2 | IMX585 | This study |
| IMX925 | MATa can1Δ::cas9-natNT2 sga1Δ::pADH1-YAT2C173G-YAT2 | IMX585 | This study |
| CEN.PK122 | MATa/MATα | P. Kötter | |
| CEN.PK194-2C | MATa cat2Δ::loxP-KanMX4-loxP | CEN.PK122 | This study |
| CEN.PK196-2C | MATα yat1Δ::loxP-KanMX4-loxP | CEN.PK122 | This study |
| CEN.PK215-4A | MATa cat2Δ::loxP-KanMX4-loxP yat1Δ::loxP-KanMX4-loxP | CEN.PK194-2C × CEN.PK196-2C | This study |
| CEN.PK113-5D | MATa ura3-52 | P. Kötter | |
| IME140 | MATa ura3-52 p426GPD (2µm ori URA3) | CEN.PK113-5D | 58 |
| IME320 | MATa ura3-52 pUDE390 (2µm ori URA3 pADH1-YAT2-tYAT2) | CEN.PK113-5D | This study |
| IME321 | MATa ura3-52 pUDE391 (2µm ori URA3 pADH1-YAT2C173G-tYAT2) | CEN.PK113-5D | This study |
| IME233 | MATa ura3-52 pUDE336 (2µm ori URA3 pTDH3-CAT2-His6-tCYC1) | CEN.PK113-5D | This study |
a
The RTG2G503T mutation translates into an Rtg2W168L protein, the MCT1T641G mutation translates into an Mct1L214W protein, and the YAT2C173G mutation translates into an Yat2P58R protein. {PDHL}, chromosomally integrated E. faecalis PDH gene cluster, pADH1-aceF-tPGI1 pPGI1-lplA2-tPYK1 pPGK1-lplA-tPMA1 pTDH3-pdhB-tCYC1 pTEF1-lpd-tADH1 pTPI1-pdhA-tTEF1. {CARN}, pTDH3-AGP2-tAGP2 pPGK1-HNM1-tHNM1 pADH1-YAT2-tYAT2 pPGI1-YAT1-tYAT1 pTPI1-CRC1-tCRC1 pTEF1-CAT2-tCAT2.