FIG 3 .

Complementation of Δrop17 and Δrop18 with the corresponding wild-type gene alleles rescues cyst burdens. C57BL/6 mice were infected i.p. with 200 tachyzoites of each strain, and brain cyst burdens were measured 21 days postinfection. Data shown are cumulative results from one to three independent experiments for each strain tested. Data on the Δrop17 and Δrop18 knockout strains and the Δku80 parent strain follow: Δku80 parent (three experiments; n = 12 mice), Δrop5 (three experiments; n = 9 mice), Δrop5::ROP5A (two experiments; n = 8 mice), Δrop5::ROP5C (two experiments; n = 8 mice), Δrop17 (two experiments; n = 8 mice), Δrop17::ROP17^FLHA (one experiment; n = 4 mice), Δrop18 (two experiments; n = 8 mice), Δrop18::ROP18 (two experiments; n = 8 mice), Δrop18::ROP18^KD (two experiments; n = 8 mice), Δrop18::ROP18^RAH2(ATF) (two experiments; n = 8 mice). The values shown represent means ± SEM. The P values were calculated with a Student’s t test. P values that were significantly different are indicated by a bar and four asterisks (P < 0.0001). P values that were not significantly different (ns) are also indicated.