Table 1. Composition of electroporation buffers.

Electroporation buffer	Composition	Electrical conductivity [mS/cm]	Osmolality [mOsm/kg]
The growth medium HAM-F12	Inorganic salts, amino acids, vitamins and other components (PAA Austria, cat. no. E15-016), 10% fetal bovine serum, L-glutamine, and antibiotics	17.14	260–320 (based on the producer’s data sheet)
The low-conductivity buffer	10 mM KH2PO4/K2HPO4 in ratio 40.5:9.5, 1 mM MgCl2, 250 mM sucrose	1.78	292
The hyperosmotic buffer	10 mM KH2PO4/K2HPO4, 1 mM MgCl2, 400 mM sucrose	1.76	475
The high-conductivity buffer	10 mM KH2PO4/K2HPO4 in ratio 40.5:9.5, 1 mM MgCl2, 150 mM NaCl	19.12	300
The buffer with calcium	10 mM HEPES, 250 mM sucrose, 0.7 mM MgCl2, 0.3 mM CaCl2	0.38	281