Table 2. Number of experiments and analyzed cells in each experiment.

Type of experiment			No. of experiments	No. of analyzed cells
Fluorescence measurement	The growth medium	4 pulses	3	19
		8 pulses	5	34
		4+4 pulses, 1 min delay	6	31
		4+4 pulses, 2 min delay	6	37
		4+4 pulses, 3 min delay	7	40
	The low-conductivity buffer	4 pulses	5	34
		8 pulses	6	45
		4+4 pulses, 1 min delay	6	38
		4+4 pulses, 2 min delay	8	55
		4+4 pulses, 3 min delay	7	48
	The hyperosmotic buffer	4 pulses	4	21
		8 pulses	3	20
		4+4 pulses, 3 min delay	4	23
	The high-conductivity buffer	8 pulses	3	24
		4+4 pulses, 3 min delay	3	23
	The buffer with calcium	8 pulses	3	21
		4+4 pulses, 3 min delay	3	25
Cell size measurement	The growth medium		4	19
	The low-conductivity buffer		7	25