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. Author manuscript; available in PMC: 2017 Jan 20.
Published in final edited form as: Nature. 2016 Jul 20;535(7612):382–387. doi: 10.1038/nature18325

Figure 1. Loss of Ptip in Brca1/2-deficient B cells protects nascent DNA from degradation without restoring HR.

Figure 1

(a) Schematic for labeling B cells with CldU and IdU. (b-e) Ratio of IdU vs. CldU upon HU treatment. Numbers in red indicate the mean and standard deviation. (ns, not significant, ****P ≤ 0.0001, Mann-Whitney test). 125 replication forks were analyzed for each genotype. (f) Genomic instability (top) and viability upon HU treatment (lower panel) relative to WT upon 6 hr of 10 mM HU treatment. (ns, not significant, ** P ≤ 0.001, *P ≤ 0.05, Unpaired t- test). 50 metaphases were analyzed. (g) Representative images (top) and quantification (below) of IR-induced RAD51 foci. (ns, not significant, *P ≤ 0.05, Unpaired t-test (n=120 cells examined)). Experiments were repeated 3 times.