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. Author manuscript; available in PMC: 2017 Jan 20.
Published in final edited form as: Nature. 2016 Jul 20;535(7612):382–387. doi: 10.1038/nature18325

Extended Data Fig. 7. Loss of RIF1 results in chromosomal instability.

Extended Data Fig. 7

(a) Quantification of RAD51 foci formation in WT, Brca1−/− and Rif1−/− B cells. Cells were treated with 10 Gy and harvested 4 hr post-IR (ns, not significant, *P ≤ 0.05). At least 100 cells were analyzed per condition; experiments were repeated 3 times. (b-c) Genomic instability measured in metaphase spreads from splenic B cells derived from WT, Brca1−/− and Rif1−/− mice treated overnight with 1 µM PARPi (b) or with 0.5 µM cisplatin (c) (ns, not significant, **P ≤ 0.01, ***P ≤ 0.001, **** P ≤ 0.0001, unpaired t-test). 50 metaphases were analyzed per condition; experiments were repeated 3 times.