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. Author manuscript; available in PMC: 2017 Jan 20.
Published in final edited form as: Nature. 2016 Jul 20;535(7612):382–387. doi: 10.1038/nature18325

Figure 2. PTIP deficiency rescues the lethality of Brca2-null mouse ES cells and confers fork protection.

Figure 2

(a) Schematic for deletion of Brca2. (b) PCR genotyping of ES cell clones (M, marker; P, positive control for Brca2f/−; N, no DNA control). (c-d) Representative images (c) and quantification (d) of IR-induced foci in shPtip and Brca2−/−/shPtip ES cells (n=110 cells examined). (e) Representative Southern blot images (top) and quantification for targeting efficiency (bottom) for 59xDR-GFP36 gene targeting to the pim1 locus. (f) Ratio of IdU vs CldU. (ns, not significant, ****P ≤ 0.0001, Mann-Whitney test). 125 replication forks were analyzed.