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. 2016 Jul 21;119(3):450–462. doi: 10.1161/CIRCRESAHA.116.308870

Figure 6.

Figure 6.

TWIST1 and GATA4 promoted permeability in endothelial cells (ECs) exposed to low shear stress. The influence of TWIST1 and GATA4 on EC permeability under low wall shear stress (WSS) was studied. A, Cells cultured on transwell inserts were exposed to orbital flow (low WSS) or static conditions for 72 h before assessment of endothelial permeability under static conditions using rhodamine (Rd) albumin as a tracer. A schematic is shown (left). The concentration of Rd-albumin in the lower compartment was measured, and mean values±SEM are shown (right). B, Human umbilical vein endothelial cells (HUVECs) were treated with 2 different small interfering RNAs (siRNAs) targeting TWIST1 or GATA4 (designated i and ii) or with scrambled nontargeting siRNA. Transfected cells cultured on transwell inserts were exposed to orbital flow (low WSS) or static conditions for 72 h before assessment of endothelial permeability using Rd-albumin. The concentration of Rd-albumin in the lower compartment was measured in 3 independent experiments, and mean values±SEM are shown. *P<0.05 using a paired t test.